Journal: Molecules

Article Title: Resveratrol Ameliorates Fibrosis in Rheumatoid Arthritis-Associated Interstitial Lung Disease via the Autophagy–Lysosome Pathway

doi: 10.3390/molecules27238475

Figure Lengend Snippet: Resveratrol treatment reversed the accumulation of P62 to enhance autophagy levels and attenuate fibrosis. ( A ) The mRNA level of LC3B was quantified. ( B , C ) The correlation between LC3B and FN1 and COL3A1 gene expression showed a stronger positive correlation. Spearman’s r and p values are indicated. ( D ) Levels of SOD2, HIF-1a, and LC3 in lung tissues were detected using Western blotting. ( E ) The immunoblotting data of LC3 II/I were quantified as described above. ( F , G ) The mRNA levels of P62 and BNIP3 were quantified. ( H ) The correlation between P62 and FN1 gene expression showed a stronger positive correlation. Spearman’s r and p values are indicated. ( I ) Levels of P62, p-P62(phospho-SQSTM1/p62(Ser349)), BNIP3, BECN1, and BCL2 in lung tissues were detected using Western blotting. ( J ) The relative intensity of P62 was quantified as described above using Western blotting. Values represent the mean ± SEM (n = 3–5). * p < 0.05 and ** p < 0.01 compared with the control group, and compared with the model group (CIA+BLM), # p < 0.05 and ## p < 0.01.

Article Snippet: Then, the membrane was sealed in 5% skim milk for 1.5 h. β-actin (Abcam, # ab8226), Collagen I (Arigo Biolaboratories, #ARG21965), HIF-1a (Proteintech, #20960-1-AP), LC3A/B (Cell signaling technology, #12741), P62 (Cell signaling technology, #16177S), Phospho-SQSTM1/p62(Ser349) (CST, #E7M1A), Bcl-2 (Abcam, #ab182858), BNIP3 (Santa Cruz Biotechnology, #sc-56167), BNIP3L (Proteintech, #12986-1-AP), BECN1 (Boster, #PB0014), and SOD2 (Boster, #BA4566) were used.

Techniques: Gene Expression, Western Blot, Control